i always thought you were supposed to put the buffer near the beginning of the chain.quoted directly from guitar effects pedals by dave hunter"a good buffer should help get your tone to the amp sounding much more like itself than that same tone passing through six pedals and many yard of wire.a good buffer can make an excellant,transparent crutch for your precious guitar signal.theres no shame in it.and if youve got eight or ten pedals in your chain,the way many players do these days,your straight,clean sound is very likely suffering badly without one."
"many,many players benifit from buffers without even knowing it.all boss and the bulk of ibanez and dod pedals contain a buffer stage,for example as do pedals from visualsound,many from roger mayer,and plenty of other quality makes,so if youve got one of these at or near the front of your pedalboard your benefiting from this already."
he goes on to say that a good buffer will drive around ten pedals.